Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae)

Centaurea amaena is an endemic and endangered species listed as CR (critically endangered) in Turkey. ISSR markers were used to detect the level of genetic diversity two natural populations C. amaena. A total 50 primers 13 producing polymorphic reproducible products selected. These yielded 102 amplified discernible loci, which 80 (78%) polymorphic. high was detected both at population levels; effective number alleles (Ne) 1.544, observed (Na) 1.784, Nei?s (H) 0.306, Shannon?s information index 0.447. The established gene flow (Nm) 2.329, indicating a migration rate between populations. moderate differentiation (GST: 0.176) also observed. Analysis molecular variance (AMOVA) revealed that 24.89% resided among populations, while 75.10% within Cluster analysis showed samples from same locality clustered together there no cross-clustering samples. patterns variation indicate existing should be conserved.